Lamp2a knockout mouse
Webb兔多克隆抗体to LAMP2A - Lysosome Marker 宿主 Rabbit 经测试应用 适用于: WB, ICC/IF, IHC-P more details 种属反应性 与反应: Mouse, Human 预测可用于: Dog, Monkey, Chinese hamster 免疫原 Synthetic peptide corresponding to Human LAMP2A aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin. LAMP2 has 3 … WebbThe NIH-3T3 mouse embryonic fibroblast cells (American Type Culture Collection, CRL-1658™) were cultured in a 37°C incubator with 5% CO 2 in DMEM medium supplemented with 10% newborn calf serum (NCS). Mouse embryonic fibroblasts (MEFs) were generated from lamp2a null mice and corresponding wild-type littermates as described previously.
Lamp2a knockout mouse
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Webb1 jan. 2024 · PLINs 2 and 3 were also found enriched in CMA+ lysosomes isolated from rat liver, and their degradation was reduced in LAMP2A knockout cells. 84 Furthermore, LAMP2A knockout cells are insensitive to starvation-induced lipolysis and display an increase in size and density of LDs. 84 Interestingly, macroautophagic activity was not …
WebbLAMP2A knockout results in a decreased CMA activity, which leads to increased PLIN2 expression, decreased lipid oxidation and subsequent ... Interestingly, SNX10 knockout mice exhibited significantly ameliorated ethanolinduced hepatic - steatosis and injury via inhibition of CTSA maturation, LAMA2A stabilization and, thus, CMA Webb7 juli 2024 · The role of chaperone-mediated autophagy (CMA) in cancer initiation and progression is not well understood due to the lack of a loss-of-function cancer models of LAMP2A, the key regulator of this process. Here, by generating an isoform-specific knockout of LAMP2A, we show that CMA deficiency promotes proliferation and tumor …
WebbLAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Webb13 nov. 2014 · Rett syndrome (RTT) is a genetic disorder characterized by a range of features including cognitive impairment, gait abnormalities and a reduction in purposeful hand skills. Mice harbouring knockout mutations in the Mecp2 gene display many RTT-like characteristics and are central to efforts to find novel therapies for the disorder. As …
Webb22 nov. 2024 · Inducible, conditional transgenic mice (Alb-Tet-off-L2A) where an exogenous copy of LAMP2a can be regulated by doxycycline have been generated to study increased CMA function in the liver . The Cre-loxP system was also used to generate conditional LAMP2a knockout mice, which have been used to study the loss of CMA …
Webb27 sep. 2024 · LAMP-2 Antibody (H4B4) is an IgG 1 κ mouse monoclonal LAMP-2 antibody (also designated LAMP2 antibody, LAMPB antibody or lysosomal associated membrane protein 2 antibody) suitable for the detection of the LAMP-2 protein of human origin by WB, IP, IF, IHC (P) and FCM. correlation versus betaWebbHuman LAMP2 knockout HeLa cell line Lysates ... Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution. Western blot - Human LAMP2 knockout HeLa cell line (ab255402) All lanes : Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker at 1/2000 dilution Lane 1 : Wild … brave words central coast writing initiativeWebbImmunohistochemical analysis of paraffin-embedded Mouse liver labeling LAMP2A with unpurified ab125068 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at … brave words bloody knucklesWebb27 apr. 2024 · Liver-specific LAMP2A-knockout mice and HepG2 cells lacking LAMP2A [L2A (–) cells] were used to investigate the influence of CMA on lipolysis in … correlation versus r2Webb21 sep. 2024 · The inhibition of CMA in young LAMP2A-knockout mice was believed to reduce the degradation of antioxidant enzymes (found to be CMA substrates), promoting ROS clearance, while old LAMP2A-knockout mice had impaired antioxidant enzymes due to disrupted protein homeostasis and were thus more susceptible to oxidative stress. 29 … brave wood splitter reviewWebbAnti-LAMP2A antibody [EPR4207 (2)] - Lysosome Marker (ab125068) Research with confidence – consistent and reproducible results with every batch. Long-term and … brave wood splitter trouble shootingWebbLAMP2A protein levels, as studied by immunohistochemistry, showed a decreased immunoreactivity in P2X7 receptor-deleted mice, having been exposed to toxin or MCD diet compared with the... brave worker is behind insolence