WebJul 22, 2016 · Acoustic shearing and sonication are the main physical methods used to shear DNA. The Covaris® instrument (Woburn, MA) is an acoustic device for breaking DNA into 100-5kb bp. Covaris also manufactures tubes (gTubes) which will process samples in the 6-20 kb for Mate-Pair libraries. WebAcoustic sample processing works by causing cavitation in the sample. Cavitation occurs in high-pressure acoustic fields where cavitation nuclei are formed in the rarefaction phase and the resulting bubble implodes on itself releasing …
GGBC Covaris
WebMar 1, 2024 · This protocol describes an automated procedure for constructing a nonindexed Illumina DNA library and relies on the use of a CyBi-SELMA automated pipetting machine, the Covaris E210 shearing instrument, and the epMotion 5075. With this method, genomic DNA fragments are produced by sonication, using high-frequency acoustic … WebApr 1, 2016 · Covaris acoustic shearing and restriction enzyme digestion showed similar LoBs and gene copy number estimates to one another. It should be noted that moderate heating, commonly used in genomic DNA extraction protocols, did not significantly increase observed KRAS mutation counts. Keywords: DNA mutation; droplet digital PCR; … buffalo shooting original live stream
M220 Focused-ultrasonicator Covaris
WebCovaris DNA/RNA Shearing. We use the Covaris S2 instrument for shearing biological material. It is similar to a probe sonicator, except that the S2 is a closed-vessel, probe … WebIt uses Covaris patented Adaptive Focused Acoustics (AFA ® ), including a spherical acoustic transducer and specifically designed AFA Tubes to deliver controlled energy to sample volumes between 15ul to 10ml. … Webacoustic shearing devices, such as the instruments made by Covaris®. This involves focused transmission of high-frequency, short wavelength acoustic energy on the DNA sample. The size of the DNA fragments generated (150 – 5,000 bp) is controlled by changing both the intensity and the duration of the acoustic waves, and the protocols crm sending bulk email to selected grouops